S. A. Rodovikov, A. A. Churakov, N. M. Popova, S. V. Khizhnyak

Soil microbial communities as a source of strains for the biological protection of soybeans against fusarium in the Yenisei Siberia

Abstract:

Eighteen strains of bacterial antagonists inhibit-ing the growth of Fusarium pathogens of soybean were isolated from soil microbial communities of the Yenisei Siberia. The two most active antagonists (RSA-1 strain, preliminarily identified as Bacillus sp., and RSA-13 strain, preliminarily identified as Streptomyces sp.) were tested as biological agents to protect soybeans from Fusarium infections in vitro. In the absence of artificial infection, the RSA-1 strain reduced the Fusarium incidence by 25 percentage points (from 61.5% to 36.5%, the statistical significance of the effect was p <0.001), and the RSA-13 strain reduced the Fusarium incidence by 32 percentage points (to 29.5%, the statistical significance of the effect p <0.001). Artificial infection of soybean with Fusarium increased the disease incidence up to 83,5% and reduced the length of seedlings by 1.9 times. In the case of artificial infection with Fusarium, the RSA-1 strain statistically significantly (p <0.001) reduced the incidence of the disease by 29 percentage points and statistically significantly (p <0.001) reduced the inhibitory effect of the infection on seedling growth. Strain RSA-13 did not affect the incidence of the disease, however, statistically significantly (p <0.001) reduced the inhibitory effect of infection on the growth of seedlings.

About the autors:

Rodovikov Sergey Aleksandrovich, ORCID: 0000-0002-4814-6493, Krasnoyarsk State Agrarian University, Krasnoyarsk, Russia, rsa12@mail.ru; Churakov Andrey Andreevich, ORCID: 0000-0002-3150-5541, Ph.D., Krasnoyarsk State Agrarian University, Krasnoyarsk, Russia, andchurakov@gmail.com; Popova Natalya Mikhailovna, Krasnoyarsk State Agrarian University, Krasnoyarsk, Russia, nmpopova@yandex.ru; Khizhnyak Sergey Vitalievich, ORCID: 0000-0003-2583-8857, Dr. habil., Krasnoyarsk State Agrarian University, Krasnoyarsk, Russia, skhizhnyak@yandex.ru

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Key words:

soybean; Glycine max (L.) Merr.; Fusarium diseases; Fusarium sp.; Yenisei Siberia; biological control