Ye. V. Nemtsova, A. V. Harin, I. A. Razlugo

THE INFLUENCE OF SILICON DIOXIDE «KOVELOS-SORB» ON GROWTH CHARACHTERISTICS OF RHODODENDRON ROSEUM (LOISEL.) REHDER CULTIVATED IN VITRO https://doi.org/10.36906/2311-4444/20-1/08

Abstract:

The article reveals a stimulating effect of synthetic amorphous silicon dioxide on growth parameters Rhododendron roseum (Loisel.) Rehder, propagating itself in vitro. The purpose of this study was to identify the optimum composition of culture medium on the basis of amorphous silica ‘Kovelos-Sorb’, used for clonal micropropagation of rhodo-dendrons. It was established that it is preferable to add to Anderson culture medium 100 mg/L of amor-phous silicon dioxide, which stimulated the growth of mericlone germs Rhododendron roseum (Loisel.) Rehder. In order to stimulate propagation and for receiving a vast amount of propagating material, it was optimal to use Anderson medium which con-tained 50 mg/L of amorphous silicon dioxide. For establishment of regenerative plants Rhododendron roseum (Loisel.) Rehder in vitro, it was optimal to use Anderson medium which contained synthetic amorphous silicon dioxide in amounts of 50–150 mg/L combined with indoleacetic acid in amounts of 1.5 mg/L.

About the autors:

Nemtsova Elena Valentinovna, ORCID: 0000-0002-6518-7799, Ph.D., Bryansk State University named after Acad. I. G. Petrovsky; Bryansk, Russia, elenanemz@mail.ru; Harin Andrey Viktorovizh, ORCID: 0002-2515-9996, SPIN-code: 2705-8104, Ph.D., Bryansk State University named after Acad. I. G. Petrovsky, Bryansk, Russia; Razlugo Irina Alekseevna, ORCID: 0000-0001-7587-777X, Bryansk State University named after Acad. I. G. Petrovsky, Bryansk, Russia.

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Key words:

Rhododendron roseum (Loisel.) Rehder; amorphous silicon dioxide; Kovelos-Sorb; clonal micropropagation.