Belkin A. V., Dubrovsky V. N., Maslakova K. Y., Turbasova N. V.

Evaluation of free radicals level in the liver and kidneys of laboratory mice when exposed to short-term supercooling

Abstract:

This work has studied the effect of short-term supercooling on the organisms of laboratory mice. The effect of low temperatures leads to the activation of oxidative processes, which are characterized by a change in the level of free-radical metabolites of oxygen in the body. Thereby, we performed a bio-chemical luminescence analysis of homogenates of the tested tissues of laboratory animals subjected to short-term supercooling. It was done in order to estimate the level of free radicals, since the intensity of bio-chemical luminescence allows determining the rate of the reaction during which these radicals are formed. The intensity of chemical luminescence is directly proportional to the rate of radical formation, thus it becomes possible to evaluate the level of free radicals that are being formed in this system. The results of our study indicate that the level of oxygen free radicals in liver and kidneys increases when laboratory mice are exposed to short-term cold stress. All cells and tissues of animals emit light in the course of their vital activity, in other words, they have so-called intrinsic chemical luminescence. However, this light is so weak that it was not possible to detect it for a long time. Therefore it was called the "superweak luminescence". This intrinsic luminescence is mainly due to the reactions involving free radicals, and measuring such luminescence is used in scientific studies aimed at laboratory analysis in the cases when it is important to detect and study the appearance of these radicals in living systems. Such low intensity of luminescence is the main obstacle for such research projects and extensive use of intrinsic chemical luminescence for analytical purposes. In this regard, the measurement of chemical luminescences in the presence of certain compounds, the so-called activators, became considerably widespread. According to the mechanism of action they are divided into two groups: chemical and physical. The action of physical activators is based on such physical process as energy transfer from the molecule of the product of chemical luminescence reaction to the activator.

About the autors:

Alexey Vasilievich Belkin (1), Candidate of Biological Sciences, Associate Professor at the Department of Human and Animal Anatomy and Physiology; Vitaliy Nikolaevich Dubrovsky (2), Candidate of Biological Sciences, Associate Professor at the Department of Human and Animal Anatomy and Physiology; Ksenia Yuryevna Maslakova (3), postgraduate at the Department of Human and Animal Anatomy and Physiology of the Tyumen State University; Natalia Vyacheslavovna Turbasova (4), Candidate of Biological Sciences, Associate Professor at the Department of Human and Animal Anatomy and Physiology

Place of employment:

Tyumen State University (1,2,4)

Contacts:

e-mail: alexbel2@mail.ru (1); e-mail: vnd3@yandex.ru (2); 71-63, e-mail: k.y.maslakova@yandex.ru (3); e-mail: turbasowa@mail.ru (4)

Key words:

stress; overcooling; bio-chemical luminescence; antioxidants; free-radical oxidation.