Kulyukina E. V., Kartashev A. G.

Effects of gasoline and diesel fuel on testate amoebae communities

Abstract:

This article presents the results of a study on the effects of gasoline and diesel fuel on the numerical and species composition of the community of testae amoebae in light gray forest soil. It has been shown that soil invertebrates can be used as bioindicators of the environment pollution level. The authors studied the effect of gasoline and diesel fuel on testae amoebae communities in laboratory conditions. Oil contaminants were added in con-centrations of 20 and 100 g/kg of dry soil. The site without pollutants was considered to be a control plot. Counting and analysis of invertebrate organisms was carried with the help of BIOMED-2 microscope with an increase of × 160. The testae amoebae accounting was made by direct microscoping of the soil suspension. Oil contamination with the concentration of 200 g / kg has the greatest impact on the structure and size of the communities. On the study sites contaminated with gasoline and diesel fuel in different concentrations, 14 species of testae amoebae were identified i.e. Phryganella acropodial, Cyclopyxis aroelloides, Plagiopyxis intermedia, Plagiopyxis glyphostoma, Cryptodifflugia compressa, Nebela dentistoma, Nebela lageniformis, Nebela collaris, Nebela militaris, Centropyxis vandeli, Centropyxis platystoma, Centropyxis cassis, Phryganella paradoxa, Cryptodifflugia minor. Testae amoebae resistant to oil products have been identified i.e. Phryganella acropodia, Cryptodifflugia compressa, Cyclopyxis aroelloides, Nebela dentistoma, Nebela lageniformis, Nebela collaris, Plagiopyxis glyphostoma. Moreover, a decrease in the number and species diversity of testae amoebae communities was recorded under different concentrations of oil contaminats in the soil. The study data can be used by researchers, teachers and students specializing in biology, general ecology and nature management.

About the autors:

Elena Viktorovna Kulyukina (1), Postgraduate at the Department of Radioelectronic Technologies and Ecological Monitoring Tomsk State University of Control Systems and Radio Electronics; Alexander Georgievich Kartashov (2), Doctor of Biological Sciences, Professor at the Department of Radioelectronic Technologies and Ecological Monitoring.

Place of employment:

Tomsk State University of Control Systems and Radio Electronics (1, 2).

Contacts:

e-mail: kuljukinaelena@rambler.ru (1), kartag@rambler.ru (2).

Key words:

testate amoebae; soil invertebrates; oil products; gasoline; diesel fuel.